North Dakota State and South Dakota State have played each other in football 99 times. This will be the first time the two schools will meet in the playoffs.
The Jackrabbits travel to Fargo Saturday to play the Bison in the second round of the Football Championship Subdivision playoffs. The winner advances to the quarterfinals.
NDSU and SDSU have become both friends and foes since joining forces for the move from Division II to Division I athletics a decade ago.
Justin Sell, the SDSU athletic director, calls it one of the "coolest rivalries" he's been a part of because the schools have many similarities, from athletic to academic programs.
The contest comes just three weeks after NDSU held off SDSU 20-17 in a game that ultimately decided the conference championship.