Florida is the overall top seed in the NCAA tournament.
The selection committee released its pairings Sunday, not long after the Gators defeated Kentucky in the Southeastern Conference tournament final to bring their winning streak to 26 games
Florida is seeded first in the South. On Thursday, the Gators play the winner of a First Four game between Albany and St. Mary's.
Kansas earned the No. 2 seed in the South and will open against Eastern Kentucky.
The Final Four starts April 5 in Arlington, Texas.
Sparked by its Atlantic Coast Conference tournament championship, Virginia is the No. 1 seed in the NCAA tournament's East Region.
The Cavaliers (28-6) will face No. 16 seed Coastal Carolina (21-12) on Friday in Raleigh, N.C. in the second round, and a win would pit them against either Memphis or George Washington.
Big East regular-season champion Villanova (28-4) earned the No. 2 seed in the East and will play Milwaukee (21-13) Thursday in Buffalo, N.Y. With a win, the Wildcats would face a familiar rival in the next round: Connecticut or Saint Joseph's.
Iowa State is the third seed and plays N.C. Central.
Other match-ups in the region include: Cincinnati-Harvard, Michigan State-Delaware and North Carolina-Providence.
The regional title game will be March 28-30 at Madison Square Garden in New York.
Arizona is the No. 1 seed in the NCAA tournament's West Region despite losing the Pac-12 championship game to UCLA.
The Wildcats (30-4) will face No. 16 seed Weber State on Thursday in San Diego and could have a tough matchup in the second round, facing the winner between Oklahoma State and Gonzaga.
Wisconsin was the No. 2 seed in the West and will open with American in Milwaukee on Thursday.
Creighton and player of the year front-runner Doug McDermott are the third seed and face Louisiana in San Antonio.
Other match-ups include Oklahoma-North Dakota State, Oregon-BYU, San Diego State-New Mexico State and Baylor-Nebraska.
The West's regional round will be Mar. 27-29 at the Honda Center in Anaheim, Calif.
Wichita State still had something to be angry about on Selection Sunday.
The second-ranked Shockers landed the first No. 1 seed in school history and will start their pursuit of a second straight Final Four on Friday in St. Louis, where they ran their record to 34-0 by winning the Missouri Valley tournament last weekend.
That's where the reward for their season ended, though. They landed in a loaded region that includes second-seeded Michigan, third-seeded Duke and fourth-seeded Louisville, the defending national champion.
Relying on their motto of "play angry," the Shockers are the first school to reach the NCAA tournament unbeaten since UNLV in 1991. They'll open against the winner of a game between Big West champion Cal Poly and SWAC champion Texas Southern.